HPLC Column, Bidentate C8 2.o, 2.2um, 2.1mm ID x 100mm Length, 120A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40208‑10P‑2 Cogent Bidentate C8™ 2.o HPLC Column is a high‑efficiency, narrow‑bore reversed‑phase column designed for applications where C18 chemistry is too retentive and additional resolving power is required. Built on Cogent TYPE‑C™ silica hydride technology, this column combines smaller 2.2 µm particles with bidentate C8 bonding to deliver excellent selectivity, faster equilibration, and near‑UHPLC performance on standard HPLC systems.
The Bidentate C8™ 2.o stationary phase provides controlled hydrophobic interaction strength, making it particularly effective for hydrophobic and non‑polar compounds that are difficult to retain in aqueous mobile phases, yet overly retained on C18 columns. The hydride surface chemistry minimizes secondary interactions and enables stable retention behavior over a wide operating window.
The 2.1 mm internal diameter supports reduced solvent consumption and enhanced detector sensitivity, while the 100 mm length increases plate count and resolving power compared with shorter formats. This combination makes the column a popular choice for LC‑MS workflows when greater separation of closely eluting components is required.
Why Choose the 2.1 × 100 mm Bidentate C8™ 2.o Format
This column size is commonly selected when:
C18 columns provide excessive retention or long run times
Additional resolution is required beyond 50 mm LC‑MS columns
Hydrophobic or non‑polar analytes require alternative selectivity
Faster equilibration and improved peak shape are desired
Coupled with TYPE‑C™ silica hydride chemistry, the C8 ligand offers a broader and more flexible reversed‑phase method space than traditional C8 columns.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Reduced silanol activity and secondary interactions
Faster equilibration than conventional silica‑based C8 phases
Excellent chemical robustness and reproducibility
Bidentate C8™ 2.o Bonding
Two‑point attachment via direct Si–C bonds
Alternative selectivity when C18 is overly retentive
Controlled hydrophobic retention with excellent efficiency
Near‑UHPLC Efficiency (2.2 µm)
High efficiency on standard HPLC instrumentation
Improved resolution and peak capacity
Suitable for demanding analytical and LC‑MS methods
LC‑MS‑Optimized Geometry
2.1 mm ID enhances sensitivity and reduces solvent use
100 mm length provides increased resolving power
Well suited for ESI‑MS and LC‑MS/MS workflows
System Requirements
Because of the narrow‑bore and small‑particle format, optimal performance requires:
A pump capable of precise, stable low‑flow delivery
A detector flow cell designed for reduced internal volume
Systems not designed for low‑flow operation may not achieve full efficiency.
Typical Applications
LC‑MS and LC‑MS/MS
Hydrophobic and non‑polar compounds
Situations where C18 retention is excessive
High‑resolution reversed‑phase separations
Method development and screening